AmplyCell provides cell line optimization services that aim to increase and stabilize the productivity of your original cell line, for recombinant protein production.
Regarding your expectations, our Amply™ technology will be applied to your specific clone or pool, in order to :
AmplyCell's Amply™ technology for cell line optimization is a solution that can be thought as a natural treatment avoiding cell culture media change, searching for cell culture parameters, or the use of gene-altering elements. Part of AmplyCell's know-how, the Amply™ technology that increase cell productivity is safe for the cells and makes no use of irradiation (UV, gamma, X-ray) or viruses or antimetabolite treatment (e.g. methotrexate). Moreover, Amply™ technology does not make use of recombination technologies to change DNA structure of the genome or the insert. Amplycell's proprietary know-how is based on a succession of physical stimulation leading to improved cell metabolism and respiratory parameters required for achieving high and sustainable expression of the protein of interest. Dozens of high-producer clones result from this stimulation process.
Sub-cloning step is performed during the cell optimization process in order to ensure monoclonality of the best clones.
Several advantages come out of cell line optimization :